Posts tagged electromicrobiology

Scientist Spotlight: Orianna Bretschger

Most of us have never thought about how to make more water or cleaner water or develop unique sources of energy but that’s exactly what Orianna Bretschger does at JCVI.  She is working at the intersection of engineering, physics, and biology to design small machines powered by bacteria that can purify wastewater and generate electricity at the same time.

Orianna Bretschger

Orianna Bretschger

Working in alternative energy was a natural career choice for Orianna since she grew up in the desert in Arizona where water was a precious and finite commodity. She also lived in places without electricity or plumbing.  One place, that holds fond memories for her, had an old-fashioned windmill that was used to pump water into an open tank for cattle.

Orianna always had an interest in science, especially astronomy, and with that interest, coupled to good teachers who always inspired her, she found her way to where she is now.

At an early age, Orianna entered the Young Astronauts program.  She had been introduced to the program by the school director, who happened to be a nun, who was at one time on the short list for the “teacher in space program.”  By junior high Orianna had attended two International Young Astronauts conferences, toured the Johnson and Kennedy Space Centers, and travelled to the former USSR with her mentors and another student to help build relationships with Young Kosmonauts; a trip that solidified her love of science.

In high school she expanded her science interests to physics and planetary science, and had the opportunity to present a poster at a Lunar and Planetary Science conference at the Johnson Space Center in Houston.  Incidentally, her teacher for these courses was also her cross-country coach – he encouraged her to go to school at Northern Arizona University, in Flagstaff, AZ (where the “dwarf planet” Pluto was discovered) where she earned a merged degree in physics and astronomy.

After her undergraduate education, Orianna went into industry, landing a job at Raytheon Missile Systems in Tucson, Arizona. There she worked on guidance systems, supporting projects in the Electro-optical subsystems department for two years, something she considers to be a great experience.  As part of her work there on the Algorithm and Analysis team she developed algorithms for the guidance systems and began the efforts to test and validate the system. As part of her job she got to fly in old military planes, collecting and analyzing flight data and system performance.

At the end of two years at Raytheon, she still had not made the decision to attend graduate school but instead headed to Authenti-Corp, a company that is involved in biometrics evaluation (e.g. facial and voice recognition devices, fingerprint and iris scanners, digital signature verification, smart cards etc.)  where her primary job was working with the Department of the Army Biometrics Task Force to develop policies and procedures for biometric systems testing and implementation. She decided that government contracting was not for her, and subsequently worked various jobs for a couple of years, including bartending in her time off, to prepare for grad school.

Orianna decided on University of Southern California, where she was accepted into the Materials Science Ph.D. program in the School of Engineering. She spent her first semester working in a Carbon Composites lab, and later met Ken Nealson in the Biology Department where she began working on microbial fuel cells (MFCs) in Ken’s laboratory.   Graduate work was focused on identifying the specific genes in the organism Shewanella oneidensis MR-1 involved with electrical current production, and solid-phase manganese and iron oxide reduction. She also led the effort to characterize the power performance, fuel flexibility, and metabolic activity of several other Shewanella wild-type strains and environmental enrichments. Throughout these projects she was given the opportunity to learn and practice microbial physiology, reactor design, electrochemistry, analytical chemistry, and environmental engineering.  She graduated in the summer of 2008 and then joined JCVI with Ken.

Orianna really enjoys the diversity of science at JCVI and according to her the wonderful people make for a terrific work environment. Specifically her interests are to extend the technology for sustainable wastewater treatment, energy recovery, and develop MFC systems for the study of microbial physiology.  Ten years from now she hopes to be tenured faculty, and believes her work with engineered and biological systems will ultimately contribute to developing healthy and sustainable water management practices throughout the Southwest and worldwide.

Note: Adapted from the original article written by Karen Nelson, Director of JCVI’s Rockville campus.

Having Fun with Genomics

I am the generation after landing on the moon. As a child, I don’t recall having any science inspiration. I was fortunate to have parents that made it possible for me and my siblings to get a very good education. I went to a small parochial school outside of Washington, DC. It was a great school but we had no labs and so my exposure to science was limited at best.  I always liked school and did well, especially in math.

Then I went to Elizabeth Seton High School and had a bumpy road of it. I had two strong teachers mentoring me, Sr. Lani, my homeroom teacher, and Sr. Mary Marguerite in Pre Calculus and Calculus. Though I still had no real strong interest in science, I decided to take AP Biology in senior year. Ms. D’Apolito made us read science journals. Wow! That was unbelievably hard!! I struggled. But in this class, Ms. D’Apolito brought her love of science and research experience into the classroom. I vividly remember reading a journal article about E. coli and thought, “This is so cool! I want to go into research.” And my friends thought I was crazy. So off to college I went, majoring in nursing.

Staying locally, I entered the Catholic University of America, planning to concentrate in nursing. My first year chemistry was taught by this incredible teacher, Dr. Diane Bunce, who is currently a Nifty Fifty with the USA Science and Engineering Festival. She made chemistry exciting and alive!! My second semester, I knew I really wanted to study science. Through many discussions with Dr. Bunce, I realized with a chemistry degree I could do anything from make new plastics to make new cosmetics (her sister worked at a major cosmetic company). Finally, I decided I really wanted Biochemistry – I love photosynthesis!! From there, I continued to work with Dr. Bunce who specialized in chemistry education. I was a teaching assistant (TA) for her professional development in the summer – working with high school chemistry teachers – and I TA’d in her non-science major chemistry class. Dr. Bunce is one of my greatest mentors.

From there, I graduated and had another extraordinary opportunity to come to work in what was described as a “controversial” lab at NIH. I had no idea what I was walking into – but am extremely grateful that Dr. Venter gave me the opportunity to work in his lab. From there, you can read Dr. Venter’s book. I participated in the greatest scientific revolution – the beginnings of genomics!! Furthermore, I have gotten back to my roots – I get to work with teachers, students and “non-science” community to excite them about science and genomics.

Why do I share my story? To explain the difference between my school science experience and the opportunities that exists today for kids of all ages. I never had the opportunity to participate in science enrichment programs because very few existed then. This coming week, JCVI is presenting at two awesome events and hosting a group of young women for a special program in San Diego. First, March 6 and 13, we will be hosting BEWiSE, Better Education for Women in Science & Engineering. BEWiSE is a program of the San Diego Science Alliance and makes a difference for talented young women who are encouraged to contribute to science and engineering professions. Twenty 9th graders will have the opportunity to explore the microbial diversity from soil in a sediment battery by working with JCVI’s Orianna Bretschger. Wow! I never did this in high school or college.

We are also presenting on March 6th at the “Expanding Your Horizon Conference” at the University of San Diego. My colleague, Crystal Snowden, will be hosting a workshop discussing the sediment fuel cell and exploring the bacteria present. This conference reaches out to young girls too! What fun to see the critters under the microscope!

Finally, we will be exhibiting along with several other organizations in the area at the San Diego Science Alliance High Tech Fair. Here, we will have the sediment batteries, microscopes for viewing sea water microbes and soil bacteria, plus slides of diatoms found in Antarctica. Did you really think that electricity could be generated from mud, let alone, sludge??

I wish I had the opportunity to go to programs like this when I was young! You never know when that spark will be lit – in a classroom, at a science festival, watching the waves or a discussion over dinner. Finally, a big thank you goes to all my mentors I’ve had in my life: Sr. Lani, Sr. Mary Marguerite, Ms. D’Apolito, Dr. Bunce and Dr. Venter!

Have you thanked your teachers and mentors lately?

Watch for pictures next week.


Many of us don’t spend a lot of time pondering wastewater treatment unless we absolutely have to.  However, we may need to start rethinking this dirty job.   In the United States wastewater treatment is a multi-billion dollar industry that is facing major challenges in the coming years including an ageing infrastructure, increasing populations, changing environmental regulations, and limited water resources.  Globally, the problem is much worse.  According to the United Nations, over 2 billion people are without access to adequate sanitation or safe drinking water; a reality that has led to devastating mortality rates among children under the age of five.  These present and future challenges demand innovative and sustainable solutions for wastewater treatment and water recycling technologies.  That is where microbial fuel cells come in…

The Electromicrobiology group at JCVI-West is developing new and innovative microbial fuel cells (MFCs) for wastewater treatment using only the metabolic action of microorganisms that naturally exist in wastewater streams.  Not only can MFC systems remove a majority of the organics contained in wastewater samples in half of the time required for traditional anaerobic systems, but they can also: (1) reduce waste-gas emissions, such as methane production; (2) significantly decrease sludge volumes (the left-over biomass and organic material from wastewater treatment); and (3) recover energy via direct electricity production.  All of these benefits lead to a water treatment system that could be deployed anywhere in the world without the need for an energy grid.

How does it work?  The answer is extracellular electron transfer.

Extracellular electron transfer results from a need to breathe.  Many microorganisms have evolved mechanisms to move electrons outside of the cellular membrane to facilitate respiration.  These mechanisms may involve the use of bacterial nanowires, outer-membrane-bound metalloproteins, and/or soluble electron shuttles.  Whatever the mechanism(s) may be, the result is an energy metabolism that allows “electrogenic” microorganisms to survive by respiring solid surfaces.  If the solid surface is the electrode of a MFC, we are able to capture some of the electrons resulting from respiration, and move them across a circuit to produce electricity.  This rapid movement of electrons away from the microbial community is what diverts energy away from methanogenesis and biomass production, resulting in a more efficient and sustainable treatment process.

By measuring electron flow across the circuit, i.e. electrical current, we are also able to directly monitor microbial respiration and use the MFCs as tools for studying electron transfer mechanisms in pure cultures and mixed consortia.   Microbial fuel cells and other bio-electrochemical systems offer many opportunities for studying microbial energy metabolism; and may also serve as unique cultivation devices.   These systems could be a useful tool for microbial enrichment and selecting for new organisms that can be sequenced and used for reference genomes.

Every bit of knowledge we gain about the fundamental processes associated with microbial energy metabolism brings us closer to optimizing MFC systems for technology applications, such as wastewater treatment.  In the Electromicrobiology group, we hope to further promote the merger between biological and engineered systems to ultimately provide MFCs that can be practically employed as cost-effective and sustainable wastewater treatment devices worldwide.

Kudos to Ken!

JCVI Professor, Kenneth Nealson, has been selected by the American Society of Microbiology to receive an award that recognizes distinguished accomplishments in interdisciplinary research and training in microbiology.  The 2010 David C. White Research and Mentoring Award will be awarded to Ken for his trail blazing work that began in the 1970s to bridge the geological and microbiological sciences.   At the JCVI he is leading the Microbial and Environmental Genomics group and established the JCVI Electromicrobiology team doing exciting research on microbial fuel cells with applications ranging from water purification to bioenergy.  Read more about Ken’s accomplishments